The regulation of rat liver xanthine oxidase. Conversion in vitro of the enzyme activity from dehydrogenase (type D) to oxidase (type O).

نویسندگان

  • F Stirpe
  • E Della Corte
چکیده

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Liver Xanthine Oxidase INVOLVEMENT OF THIOL GROUPS IN THE CONVERSION OF THE ENZYME ACTIVITY FROM DEHYDROGENASE (TYPE D) INTO OXIDASE (TYPE 0) AND PURIFICATION OF THE ENZYME By E. DELLA CORTE and F. STIRPE

1. The 'xanthine oxidase' activity of rat liver supernatant, most of which behaves as an NAD+-dependent dehydrogenase (type D) can be rapidly converted into an oxidase (type 0) by thiol reagents such as tetraethylthiuram disulphide, copper sulphate, 5,5'-dithiobis(2-nitrobenzoic acid), N-ethylmaleimide and p-hydroxymercuribenzoate. Treatment with copper sulphate, if prolonged, leads to almost c...

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Allopurinol, the xanthine oxidase inhibitor, is the only drug available for the treatment of gout. We examined the xanthine oxidase inhibitory activity of some commercially available flavonoids such asepigallocatechin, acacatechin, myricetin, naringenin, daidzein and glycitein by virtual screening and in-vitro studies. The interacting residues within the complex model and their contact types we...

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Onion (Allium Cepa) contains high levels of flavonoids. Although there are many studies indicating the inhibitory effects of flavonoids on xanthine oxidase, there is no report on the effect of onion on this enzyme. Therefore, in the present study, the inhibitory effects of onion on xanthine oxidase are investigated. Fresh filtered juice of onion was prepared and its inhibitory effect on guine...

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عنوان ژورنال:
  • The Journal of biological chemistry

دوره 244 14  شماره 

صفحات  -

تاریخ انتشار 1969